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. 2016 Jul 12;44(18):8786–8798. doi: 10.1093/nar/gkw626

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Covalent capture of thio-phosphorylated peptides by AS-CDC7. (A) In vitro kinase assay with recombinant WT or M118A, M134A and M118A/M1134A CDC7/DBF4, recombinant MCM2 and N⁶-(benzyl)ATP-γ-S. Thio-phosphorylated MCM2 and CDC7 proteins are detected by western blotting. Ponceau-stained membrane shows equal amount of MCM2 substrate in reactions. (B) Western blot detection of thio-phosphorylated proteins after kinase reaction with nuclear extracts and N⁶-(benzyl)ATP in the presence or absence of AS-CDC7. (C) List of proteins and peptides identified after covalent capture of thio-phosphorylated peptides. The sites of modification are indicated in red.