Figure 2.

CDC7/DBF4 interacts with TOP2A. (A) Proteins extracted from nuclei with or without Benzonase were analysed by western blotting with the indicated antibodies. (B) Immunoprecipitations (IP) with anti-FLAG antibodies were performed from T-REx-EV and T-REx-DBF4 cells. (C) Schematic of DBF4 deletion mutant alleles used to generate Flp-In T-REx 293 conditionally expressing cell lines. (D) Detection of DBF4 fragments by western blotting in nuclear extracts prepared from Flp-In T-REx 293 cells upon addition of doxycycline. Asterisks indicate cross-reactive bands. (E) Endogenous TOP2A was immunoprecipitated from nuclear extracts and immunoprecpitated material was analysed by western blotting.