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. 2016 Jul 12;44(18):8786–8798. doi: 10.1093/nar/gkw626

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — CDC7/DBF4 interacts with TOP2A predominantly in early S-phase. (A) T-REx-DBF4 cells were treated with mimosine or nocodazole for 19 h and DNA content analysed by Flow Cytometry. (B) Extracts were prepared from asynchronously growing, mimosine and nocodazole treated cells as in panel A and TOP2A was immunopreciptiated with specific antibodies. As control, unrelated mouse IgG was used. Immunoprecipitated proteins were then analysed by western blotting. (C) DNA content of cells treated with mimosine for 15 h and released into fresh media. Samples were collected at the indicated time-points. (D) TOP2A immunoprecipitates from extracts of cells released from mimosine block as in panel C.