Figure 6.

Complex lytic promoter usage for LMP2 transcripts. (A) Genome browser visualization of CFLs mapping to the LMP2 exons in induced Akata cells and LCLs. Arrows positioned at the beginning of reads signify those with validated 5′ ends. (B) Splice junction read depth for SMRT circular consensus and Illumina short-read sequencing. Labels A through E refer to junctions indicated below GenBank-annotated gene track in (A). (C) PCR using junction-spanning primers in Akata, Mutu, JY and X50-7 cells. Akata + αIgG and Mutu + αIgM refer to Akata and Mutu cells induced for 24 and 48 h, respectively. (D) qRT-PCR of nuclear and cytoplasmic fractions of induced Akata cells (24 h).