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. 2016 Aug 5;44(18):8897–8907. doi: 10.1093/nar/gkw699

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — A schematic phylogenetic tree of RlmN and Cfr sequences from selected Firmicutes species. Known Cfrs, known and putative RlmNs and Cfr-like sequences are marked. Underlined with a continuous black line are the enzymes characterized in this study: RlmN from B. subtilis (Bsub_RlmN) that is part of the Bacillus clade, and a Cfr-like from C. phytofermentas (ClCp) in the divergent clostridial clade. A discontinued underline indicates the previously characterized Cfr-like enzyme from C. sporogenes (27). An asterisk specifies a functionally uncharacterized enzyme from B. methylotrophicus that clades with known Cfr enzymes. A Paenibacillus species that possesses two putative RlmN paralogues is shown, and the paralogues that appear to belong to two separate Cfr clades are marked as I and II. Two putative paralogous RlmNs from Staphylococcous species that belong to these clades are also marked. The non-collapsed version of the phylogenetic tree with the full names and IMG/JGI database identifiers is shown in Supplementary Figure S8.