Fig. 3.

CagL C-terminal coiled-coil region is involved in H. pylori-adhesion. (A + C) AGS grown on chamber slides were stimulated with H. pylori B128 WT and isogenic H. pylori B128 ΔcagL, B128ΔcagL(1-206) and B128ΔcagL(1-224) mutant strains. The AGS were stained with phalloidin (red), the bacteria detected with anti-H. pylori antibody stained with FITC-labeled secondary antibody (green), and merged at 1:100 and 1:400 magnification (yellow: overlap of bacteria and cells). (B) Quantification of adherent H. pylori strains (B128 WT and isogenic B128 ΔcagL, B128ΔcagL(1-206) and B128ΔcagL(1-224) mutants) to AGS. The mean values ± SEM of at least three independent experiments are shown. Statistical analysis (Student’s t test) was applied using SigmaStat software. ^***P ≤ 0.001. (C) AGS stimulated with H. pylori strains were detected as described above for “hummingbird” phenotype appearance