Table 2.

ipaH and lacY primers and probes used in the present study

Gene	Primer or probe*	Sequence (5′–3′)	Melting point (°C)	PCR product (bp)	Fluorochrome (5′ end)	Reference
lacY†	lacY-F	ACCAGACCCAGCACCAGATAAG	59	104		[19]
	lacY-R	TTCTGCTTCTTTAAGCAACTGGC	58.9			Modified from [19]
	lacY-MGB-p1	CATACATATTGCCCGCCAGTA	70		FAM	Modified from [19]
	lacY-MGB-p2	CATACATATGCCCGCCAGA	70		FAM	Modified from [19]
ipaH	ipaH-F	GACGGACAACAGAATACACTCCATC	59.8	108		Modified from [22]
	ipaH-R	ATGTTCAAAAGCATGCCATATCTGT	59.8			[22]
	ipaH-MGB-p	CGGAAAACAAACAATCTGATGT	69		VIC	Modified from [22]

^*All probes were conjugated with minor groove binder (MGB) and had a “Black Hole Quencher” at the 3′ end

^†Due to sequence variation in the lacY gene of certain EIEC strains, two different lacY probes were used to detect all EIEC strains [19]