Table 4.
Results of the five different nucleic acid purification kits in combination with the three subsequent real-time PCR approaches
| 16S PCR | rpsU PCR | BpTTS1 PCR | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ki | Li | Lu | Br | He | Sp | Ki | Li | Lu | Br | He | Sp | Ki | Li | Lu | Br | He | Sp | ||
| QIAamp DNA FFPE Tissue Kit | M1 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| M2 | – | – | – | – | – | – | + | – | – | – | – | + | – | + | – | – | – | + | |
| M3 | – | – | – | – | – | – | – | – | – | – | – | – | / | / | / | / | / | – | |
| M5 | – | / | – | – | – | – | – | / | – | – | – | + | – | / | – | – | – | + | |
| M7 | – | / | – | – | – | – | – | / | – | – | – | – | – | / | + | – | + | + | |
| M10 | – | – | – | – | – | – | – | – | – | + | – | + | – | – | – | – | – | + | |
| M15 | / | / | / | – | – | / | / | / | / | – | – | / | / | / | / | + | + | / | |
| QIAamp EZ1 DNA Tissue Kit | M1 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | + |
| M2 | – | – | – | – | – | – | – | – | – | – | – | + | + | + | – | – | – | + | |
| M3 | – | – | – | – | – | – | – | – | – | – | – | – | / | / | / | / | / | – | |
| M5 | – | / | – | – | – | – | – | / | – | – | – | + | – | / | – | + | – | + | |
| M7 | – | / | – | – | – | – | – | / | – | – | – | – | + | / | + | + | + | + | |
| M10 | – | – | – | – | – | – | – | – | – | – | – | + | – | – | – | – | – | + | |
| M15 | / | / | / | – | – | / | / | / | / | – | – | / | / | / | / | – | – | / | |
| QIAamp DNA Mini Kit | M1 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | + |
| M2 | – | – | – | – | – | – | – | – | – | – | – | + | – | – | + | – | – | + | |
| M3 | – | – | – | – | – | – | – | – | – | – | – | – | / | / | / | / | / | – | |
| M5 | – | / | – | – | – | – | – | / | – | – | – | + | – | / | – | + | – | + | |
| M7 | – | / | – | – | – | – | – | / | – | – | – | + | + | / | + | + | + | + | |
| M10 | – | – | – | – | – | – | – | – | – | – | – | + | – | – | + | – | – | + | |
| M15 | / | / | / | – | – | / | / | / | / | – | – | / | / | / | / | – | + | / | |
| QIAamp DNA Blood Mini Kit | M1 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | + |
| M2 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | + | |
| M3 | – | – | – | – | – | – | – | – | – | – | – | – | / | / | / | / | / | – | |
| M5 | – | / | – | – | – | – | – | / | – | – | – | + | – | / | – | – | – | + | |
| M7 | – | / | – | – | – | – | – | / | – | – | – | + | + | / | + | – | – | + | |
| M10 | – | – | – | – | – | – | – | – | – | – | – | + | – | – | – | – | – | + | |
| M15 | / | / | / | – | – | / | / | / | / | – | – | / | / | / | / | – | – | / | |
| QIAamp FlexiGene DNA Kit | M1 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | + |
| M2 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | + | |
| M3 | – | – | – | – | – | – | – | – | – | – | – | – | / | / | / | / | / | – | |
| M5 | – | / | – | – | – | – | – | / | – | – | – | – | + | / | – | + | – | + | |
| M7 | – | / | – | – | – | – | – | / | – | – | – | – | + | / | + | – | – | + | |
| M10 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | + | |
| M15 | / | / | / | – | – | / | / | / | / | – | – | / | / | / | / | – | – | / | |
+ = positive results, – = negative results, / = no samples