Fig. 7. Alternative enrichment methods to biotin–streptavidin. (a) IAF (immunoaffinity fluorescent tag) reported by La Clair et al. for antibody-based enrichment of probe-bound proteins. (b) Click-on-resin approach reported for the NP (natural product) scalaradial: scalaradial 2 is functionalised to generate probe 3, which can be attached in turn by CuAAC to an alkyne-functionalised resin.45 (c) On-resin cleavable linker reported by Sibbersen et al. 46 .
