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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Mol Microbiol. 2016 Apr 15;100(6):1039–1065. doi: 10.1111/mmi.13366

Fig. 2.

Fig. 2

Growth curves and Boc-FL labeling of PBPs in reconstructed Δpbp2b sup2-5 strains and growth curve of the mltGSpn-Eco chimera mutant strain. The mutations are illustrated in the middle and at the bottom of Figure 1. Reconstruction of Δpbp2b sup 2-5 is diagramed in Figure S4 and described in Table S1. Reconstructed Δpbp2b sup2-5 strains are: IU9777 (mltG(Δ5bp) (sup2) Δpbp2b); IU9783 (mltG(Y488D) (sup3) Δpbp2b); IU9905 (mltG(Δ488bp) (sup4) Δpbp2b); and IU9907 (mltG(Ω45bp) (sup5) Δpbp2b). (A) Representative growth curves of isogenic pbp2b+ parent strain IU1824 and reconstructed Δpbp2b sup2-5 strains. Strains were grown as described in Experimental procedures. (B) Fluorescent-bocillin (Boc-FL) binding to PBPs in isogenic strains IU6741 (Δpbp1a), IU7850 (Δpbp1b), IU7852 (Δpbp2a), IU1824 (wild-type parent) and the reconstructed Δpbp2b sup2-sup5 strains listed above was performed as described in Experimental procedures. (C) Growth curves of S. pneumoniae strains IU1824 wild-type parent (D39 Δcps rpsL1), IU10919 (IU1824 mltGSpn-Eco), and IU11007 (mltGEco) (see Table S1). Growth curve determinations and Boc-FL binding experiments were performed three times independently with similar results. See text for additional details.