TABLE 1.
Mutation in D39 Δcps Δpbp2b suppressor mutants determined by Illumina whole-genome sequencinga
| Strainb | Gene and mutationc | Function | nucleotide changed |
|---|---|---|---|
| 1. sup2 (IU7477) | spd_1346 (mltG) (Δ5 bp at Y403) | Hypothetical YceG-like family protein | TACTATAG→TAG (Δbp 1212–1216/1656, Y403stop) |
| 2. sup3 (IU7567) | spd_1346 (mltG) (Y488D) | Hypothetical YceG-like family protein | TAT→GAT (bp 1462/1656) |
| 3. sup4 (IU7570) | spd_1346 (mltG)( Δ488 bp at T54) | Hypothetical YceG-like family protein | Internal out-of-frame Δ488 bp at 165–652/1656 |
| groES (upstream) | Co-chaperonin GroES | G→T at −48 | |
| 4. sup5 (IU7765) | spd_1346 (mltG) (Ω45 bp)2 | Hypothetical YceG-like family protein | Out of frame 45 bp (bp 302–346) duplication, TCT→TGA (S101stop) at bp 301–303 |
| spd_0115 (G41C) | HlyD family secretion protein | GGC→TGC (bp 121/1253) |
a
Illumina whole-genome sequencing was performed as described in Experimental procedures.
b
Strains IU7477, IU7567, IU7570 and IU7765 were isolated from the small number of colonies that arose following independent transformations of unencapsulated D39 Δcps strain IU1945 with a Δpbp2b<>aad9 amplicon (see text and Table S1).
c
Besides the Δpbp2b<>aad9 insertion, the mutational changes shown are compared to the sequence of the IU1945 parent strain.
d
Nucleotide and codon changes are indicated by underlined and bold letters, respectively.