Figure 1.
Lack of Usp9x results in the upregulated mRNA expression levels of oligodendrocyte and myelin associated genes within the hippocampus at P14. (A) Signal intensity plot showing the differentially expressed genes for comparison between Usp9xloxP/Y control mice vs. Usp9x−/Y; Emx1-Cre mutant mice. Only the genes that were statistically (p < 0.05) and magnitudually (log2 ratio fold change <−0.5 or > 0.5) differentially expressed were colored. Upregulated genes are illustrated in red and downregulated genes are illustrated in green. All other genes are shown in gray. The dotted diagonal line shows equal intensity across the experimental conditions while the solid lines correspond to a log2 ratio fold change of <−1 or > 1. (B) Summary of the microarray results, showing the gene symbol, gene name, log2 ratio fold change, and p value of key significantly misregulated genes in the hippocampus of mutant mice at P14. (C) qPCR validation of the microarray results, demonstrating increased relative levels of oligodendrocyte and myelin associated mRNAs, as well as reduced levels of neurogenic mRNAs, in the hippocampus of mutant mice at P14. *p < 0.05; **p < 0.01; ***p < 0.001, t-test.