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. 2014 Oct 30;5(3-4):99–102. doi: 10.4161/19381956.2014.959827

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© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — Approach used for detection of maternal MC cells among fetal cells by flow cytometry. Living cells were detected in lymph nodes from C57Bl/6 females (A), bone marrow of BALB/c males (B) and in fetal thymus and bone marrow in offspring of BALB/c-mated C57Bl/6 females (C), based on their 7AAD negativity (not shown) and gated for CD45 positivity (left panel). Within the respective CD45⁺ gates (solid squares), the frequency of cells expressing H-2D^b or H-2D^d was detected (right panel). Maternal MC was identified based on the frequency of CD45.2⁺H-2D^b cells among CD45.2⁺1⁺H-2D^b/d fetal cells (stippled squares in C, right panel).