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. Author manuscript; available in PMC: 2017 Oct 17.
Published in final edited form as: Vaccine. 2016 Sep 20;34(44):5235–5242. doi: 10.1016/j.vaccine.2016.09.012

Fig. 2.

Fig. 2

Indirect immunofluorescence assays (IFA) for Sf9 cells infected with recombinant baculovirus (rBV) expressing H5, H7, H9, H10, NA, and Bgag genes. Aliquots of the infected cells (upper panel) and uninfected controls (bottom panel) were seeded into chamber slides and incubated for 48h. Cell monolayers were fixed with cold acetone and probed with primary antibodies specific for H5, H7, H9 or H10 antigens as indicated. After incubation, monolayers were probed with appropriate species-specific, FITC-labeled antibodies to visualize expressed HA antigens (in green color). Slides were covered with mounting medium containing propidium iodide counterstain to visualize nuclei (in red).