Figure 3. Loss of Rhox10 Causes SSC Defects.

(A) Representative image of whole mount staining of 6 month-old adult mice seminiferous tubules with GFRα1 antibody. A_s, A_pr and A_al4 cells are positively stained with the GFRα1 antibody.

(B) Quantification of GFRα1⁺ spermatogonia in testes from 6- to 10-month-old Rhox10-KO and littermate control (WT) mice (n=4-5 per genotype). All values are mean ± SEM. Asterisks indicate differences that are statistically significant (P<0.05).

(C) Germ cell transplantation analysis. Donor-derived colonies in recipient testes were counted and normalized to 10⁵ cells injected per testis 3 month after transplantation of testicular cells from P7-8 Rhox10-KO (Rhox10^−/y;Ubc-Gfp) mice (n=4) and littermate control (WT) (Rhox10^+/y;Ubc-Gfp) mice (n=5).

(D) Germ (TRA98⁺) cells per tubule in testes sections from Rhox10-KO and littermate control (WT) mice of the indicated ages (n=1-4, each time point).

(E) Cell cycle analysis of either total testicular cells or Id4-eGfp⁺ cells from P5 Rhox10-KO and littermate control (WT) mice (n=5).

(F) Flow cytometric analysis of Annexin V⁺ cells (among total, Id4-eGfp⁺ or Id4-eGfp^high testicular cells) from P5 Rhox10-KO mice (n=6) and littermate control (WT) mice (n=9).

(G) Quantification of STRA8⁺ cells in testes sections from Rhox10-KO and littermate control (WT) mice of the ages indicated (n=2-5, each time point). All values are mean ± SEM.

See also figure S3