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. 2015 Nov 12;18(4):573–590. doi: 10.1111/cmi.12535

Figure 2.

Figure 2

Effect of human complement on ABS replication and gametocytogenesis.

A. Effect of human complement on ABS replication. Ring stage cultures of strain NF54 with a starting parasitemia of 0.5% were maintained in cell culture medium supplemented with either 10 vol% NHS or HIS at 37°C over a period of 0–120 h to follow three generations (G1–3). The parasitemia was evaluated every 12 h via Giemsa smears. Significant differences in parasitemia are indicated (* p < 0.05, *** p < 0.001; Bonferroni post‐test, two‐way ANOVA). The experiment was performed in triplicate (mean ± SD); the data are representative for one of two independent experiments.

B. Effect of human complement on gametocytogenesis. Ring stage cultures of strain NF54 with a starting parasitemia of 5% were maintained in cell culture medium supplemented with either 10 vol% NHS or HIS at 37°C over a period of 0–240 h. The gametocytemia was evaluated every 24 h via Giemsa smears. Significant differences in gametocyte numbers are indicated (** p < 0.01, *** p < 0.001; Bonferroni post‐test, two‐way ANOVA). The experiment was performed in triplicate (mean ± SD); the data are representative for one of two independent experiments.