Table 1.

LC₅₀ estimates for two laboratory susceptible strains of S. frugiperda larvae exposed to Vip3Aa19 insecticidal protein when tested over multiple generations

Generation	SUS				K‐SUS
	Number of insects tested	LC50 (95% CL)a	Slope	SE	Number of insects tested	LC50 (95% CL)a	Slope	SE
1	NAb	NA	NA	NA	380	22.5 (17.5–27.9)	3.55	0.42
2	380	11.9 (10.0–13.8)	4.25	0.59	NAb	NA	NA	NA
3	767c	8.7 (6.9–10.4)	2.7	0.35	767c	46.3 (38.2–54.6)	3.2	0.54
4	381	12.6 (10.3–15.0)	3.43	0.45	381	22.4 (19.2–25.8)	4.3	0.54
5	384	20.7 (17.5–24.0)	4.36	0.6	331	19.6 (14.8–24.4)	4.8	0.69
6	508	36.2 (27.4–48.2)	3.32	0.3	508	83.2 (59.3–126.9)	1.67	0.24
7	512	∼80d	NC	NC	508	21.7 (17.0–26.0)	3.2	0.44
8	507	17.5 (11.1–23.7)	2.95	0.42	511	∼40d	NC	NC
9	512	28.8 (18.8–45.5)	2.59	0.2	504	33.7 (19.0–63.3)	2.39	0.2
10	511	38.3 (29.8–47.6)	3.08	0.34	511	40.4 (35.6–45.1)	4.98	0.64
11	511	23.2 (20.5–26.1)	4.34	0.46	510	36.6 (32.0–41.6)	3.8	0.4
12	510	26.7 (20.8–32.6)	3.31	0.37	508	34.8 (24.1–49.9)	4.73	0.48
13	511	23.8 (21.0–26.8)	3.97	0.38	511	19.2 (16.3–22.2)	3.16	0.33
14	512	51.2 (38.8–66.8)	3.38	0.33	1017e	28.1 (22.2–34.6)	2.5	0.18
15	510	27.5 (11.2–42.9)	2.98	0.37	511	11.6 (9.8–13.3)	4.05	0.51
16	500	30.8 (22.3–39.3)	3.4	0.42	512	33.0 (27.8–38.5)	2.94	0.29
17	511	54.3 (46.8–60.9)	5.02	0.79	507	102.2 (72.8–129.1)	4.4	0.87

^ang Vip3Aa19 cm⁻² diet.

^bData not available (NA) for this generation, as no bioassay was conducted.

^cSix replicates used at this generation testing.

^dThe LC₅₀ value was not calculated (NC) by probit analysis and was estimated on the basis of 50% observed mortality.

^eEight replicates used at this generation testing.