Fig. 4. Pyrrophenone inhibits translocation of the cPLA2α C2 domain in serum-stimulated IMLFα.
IMLFα−/− expressing the cPLA2α C2 domain were pre-treated with 2 µM pyrrophenone or vehicle (DMSO) for 30 min and then stimulated with serum. (A) Representative images show (left panel) translocation of EYFP-C2 domain to Golgi (arrow) 30 sec after stimulation with serum. (B) Graphical representation showing EYFP-C2 domain translocation to Golgi over time after stimulation with serum in two independent experiments (E1, E2) from analysis of at least 5 cells/experiment.