Table 1. Primer and probe sequences of the quadruplex assay.
| Target gene (species) | Oligo | Sequence (5’ → 3’) | Amplicon Size (bp) |
|---|---|---|---|
| 16.5 kDa | |||
| B. mallei | Forward | CGT TCG AGC TCA GCA ACC TCG TTA | 85 |
| Reverse | AAG CGG TGA TGG ACC GCT GTA T | ||
| Probe | Cy5 -CAG TAT CCA GGT TTC ACC GCG CTC GAC-IAbRQ | ||
| Orf11 | |||
| B. pseudomallei | Forward | AAC ACT GAC AAG TGG CCC TAT GGA | 185 |
| Reverse | TCC GAT CGG TTT CGA ATA ACG GGT | ||
| Probe | FAM -ACG ATC TCC-ZEN-GAG AAC GCA CTG AAC A-IAbFQ | ||
| 70 kDa | |||
| B. thailandensis | Forward | AAC CTG AGG CAA CGC AAG AAG AAG | 99 |
| Reverse | TTT CTT CAC GCA TTC CCA ACC CTG | ||
| Probe | Tex615 -TCA AGG CGA GCT GTG CCG ACA ACA A-IAbRQ | ||
| fliC | |||
| Bp, Bm, & Bt group | Forward | ACG GTC AAC AAY CTG CAG GCA A | 143 |
| Reverse | TTC GCG GTT TCC TGA GCR AAG TC | ||
| Probe | Cy3- GGC TCG AAC AAC CTC GCG CAR G-IAbRQ | ||
IAbRQ, Iowa Black RQ Quencher; IAbFQ, Iowa Black FQ Quencher; ZEN, ZEN Internal Quencher; Bp, B. pseudomallei; Bm, B. mallei, Bt, B. thailandensis