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. 2016 Oct 13;11(10):e0163215. doi: 10.1371/journal.pone.0163215

Fig 1. Detection of small RNA molecules homologous to AfuSINE sequences.

Fig 1

Total RNAs isolated with Trizol were electrophoresed on 1.5% agarose gels (a). After separation by PEG precipitation, high molecular weight RNAs (HMW RNAs) and low molecular weight RNAs (LMW RNAs) were electrophoresed on 1.5% agarose gels (b). The LMW fractions from 400 ng/μl of total RNA were resolved on 15% (w/v) polyacrylamide Tris-borate-EDTA-urea gels and stained with SYBR Gold Nucleic Acid Gel Stain (c). Northern blot hybridization of small RNAs homologous to AfuSINE sequences in A. fumigatus Af293 (d). LMW RNA fractions were isolated using TRIzol and 5 μg RNA sample was loaded into each well for northern blot analysis. Only small RNAs (<40 nt; arrowed) from the antisense strand were detected.