Fig 1. Transcriptional activity of alternative GR variants harboring the Ala610Val substitution after treatment with different concentrations of steroid.

Presented are dose-response curves and EC50 estimations for the endogenous ligand cortisol in a wide (A) and narrow range (B). Additionally, the transactivation activity of aldosterone (C) and progesterone (D) is illustrated. The wild-type variant is labeled as Ala610 (blue) and the mutant GR variant as Val610 (red). Plotted are normalized means ± SEM of two separate experiments performed in triplicate.