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. 2016 Oct 13;11(10):e0164628. doi: 10.1371/journal.pone.0164628

Fig 2. Cytosol-to-nucleus shuttle and subcellular localization of GFP-tagged GR variants.

Fig 2

Cos-7 cells were transiently transfected with either the wild-type Ala610 (A) or the mutant Val610 (B) GR variant and stimulated with 10 nM dexamethasone to induce translocation. Representative images of time-series showing the same cells are presented (A and B). The increase in fluorescence intensity in the nucleus after dexamethasone stimulation (C) and the comparison of the estimated half-maximal translocation time between both GR variants (D) based on observations of three independent transfection experiments are depicted.