Fig 4. GroEL induces DNA fragmentation.

PBMCs were treated with AaGroEL (250 ng) for 72 h. RPMI alone was used as a negative control. At the end of the incubation, the cells were stained using the MEBSTAIN apoptosis detection kit and analyzed by Olympus fluorescence microscopy (magnification x20). Cells with fragmented DNA were positively labeled with FITC-conjugated dUTP.