Fig 5. PCF labelling does not affect the function of total splenocytes (SP), anti-CD3/anti-CD28 activated (Tact) and Ova-peptide activated (TOVA-act) splenocytes from OT-1 mice.
CD107a upregulation (A) and IFNɣ production (B) by CD3+CD8+ T cells were analyzed after co-culture of corresponding cells with B16-OVA mouse melanoma cells, used as antigen presenting cells. (C) Cytotoxicity of corresponding cells in same conditions was measured by the incorporation of red reagent in dead cells. The acquisition was done by Incucyte microscope. (n = 3 for each group of stimulation)