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A. The TreadWheel holds a maximum of 48 vials, and each clamp is removable. The unit features an adjustable rotation speed, with these experiments using 4RPM. B. The distance between the plugs at the top and bottom of the vial for each vial was adjusted to 6cm for the exercised flies and to 1cm for the control flies. These vials were then clipped into the TreadWheel for the exercise regime.
