Figure 2.

Abnormal Mitochondria in PD iPSC-Derived Midbrain Neuronal Population

(A) Electron microscopy photographs taken at day 75 of differentiation. Lower panels are enlarged images of the corresponding boxed area marked in upper panels. Scale bar, 500 nm.

(B) Percentage of abnormally shaped mitochondria among all mitochondria in a given midbrain neuronal population.

(C) Relative mitochondria size for each line compared with the average size measured in H9-derived cells (n = 20–50; mitochondria from two independent preparations). Significance levels for individual markers are presented as comparisons with the H9 wild-type group: ^∗p < 0.01, ANOVA and Dunnett’s test.

(D) Live staining image of MitoSOX indicating oxidative mitochondria. Black and white images in the upper panels and red color in the lower panels represent MitoSOX labeling, and blue (lower panels) represents Hoechst for DNA. Scale bar, 50 μm.

(E) Quantification of relative fluorescence intensity of MitoSOX by image analysis (n = 3; independent experiments). Significance levels for individual markers are presented as comparisons with the H9 wild-type group: ^∗p < 0.001, ANOVA and Dunnett’s test.

All data are presented as mean ± SEM. See also Figure S3.