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. Author manuscript; available in PMC: 2016 Nov 23.
Published in final edited form as: Nature. 2016 May 23;534(7605):115–118. doi: 10.1038/nature17955

Extended Data Figure 10. CNO activates cells with DREADD receptors and does not increase anxiety in aged mice.

Extended Data Figure 10

a, Mice infected with DREADD virus in CA1 were injected with saline (SAL) or clozapine-N-oxide (CNO) and then sacrificed 90 minutes post-injection for immunofluorescence staining. b, There was no difference in the percentage of DREADD-positive cells (labeled with GFP) between SAL and CNO groups (unpaired t-test, t7 = 0.01, n.s., n=3,6). c, DREADD-positive cells (labeled with GFP) had more ZIF when injected with CNO than SAL (unpaired t-test, t7 = 5.08, p=002). d, Representative examples of ZIF, DREADD, DAPI as well as merged images of CA1. e, Design for elevated plus maze experiment in aged mice with DREADD virus. f, A two-way ANOVA showed no main effect of injection (F1,9 = 0.75, n.s., n=6,5) and a significant main effect of arms (F1,9 = 71.03, p<0.0001). There was no significant interaction between injection and arms (F1,9 = 0.003, n.s.). Results show mean +/− sem.