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. Author manuscript; available in PMC: 2016 Nov 23.
Published in final edited form as: Nature. 2016 May 23;534(7605):115–118. doi: 10.1038/nature17955

Figure 2. Tagging neural ensembles of contextual memories with the TetTag system.

Figure 2

a, Schematic design of the TetTag system. b, Experimental design. Cells active in context A were tagged with GFP and cells active in context B, either 5h or 7d later, were labeled with ZIF immunohistochemistry. c, Representative examples of GFP, ZIF, DAPI and merged images of CA1. d, There was no difference between the percent of cells positive for GFP (unpaired t-test, t24=0.54, n.s., n=15,11). e, There was no difference between the percent of cells positive for ZIF (unpaired t-test, t24=1.11, n.s., n=15,11). f, There was an increase in the overlapping ensemble between contexts when spaced 5h apart compared to 7d apart (unpaired t-test, t24=2.15, p=0.0422, n=15,11). The level of the overlapping ensemble for the 5h group was above chance (one-sample t-test against 0, t14=3.402, p=0.0043) and at chance for the 7d group (one-sample t-test against 0, t10=0.323, n.s.). Significance: *p<0.05. Results show mean +/− sem.