Table 2.

Percentage hydrolysis of 1 mg P L^− 1 organic phosphorus and inorganic polyphosphate compounds by five different enzymes or enzyme combinations, at a final concentration ranging from 0.001 to 0.1 units mL^− 1 solution, in either acetate or tris buffer.

Enzyme	Phosphatase (potato)		Phosphatase (Escherichia coli)				Phosphatase (bovine)		Phosphatase (Escherichia coli) + nuclease		Phytase (wheat)
Buffer	Acetate, pH 5		Acetate, pH 5			Tris, pH 8	Acetate, pH 5		Acetate, pH 5		Acetate, pH 5		Tris, pH 8
Enzyme concentration (units mL− 1)	0.01	0.1	0.001	0.01	0.1	0.1	0.01	0.1	0.01	0.1	0.01	0.1	0.01	0.1
Phosphomonoesters
Cytidine monophosphate	20	95	20	103	102	102			98	98	10	96	15	56
p-NPP	101	93			103	99	95	113	94	99	7	95	87	102
Glycerol phosphate	70	96					95	124	100	105	0
Glucose-1-phosphate							97	102	88	109
Glucose-6-phosphate	52	95	26	103	99		81	98	98	108	4	95	18	63
Inositol hexakisphosphate	0	7		2	63		21	80	4	2	13	109	84	105
Phosphodiesters
bis-p-NPP	35	95			1	1	10	49	0	0	15	98	14	83
DNA	0	13			4	1	0	6	74	86	12	95	28	91
RNA	10	81			14	14			80	87	7	91	29	72
Polyphosphates
Sodium pyrophosphate	111	104			113	63	113	119	95	90	0	107	98	116
Sodium hexametaphosphate	85	96			106		48	102	102	107	4	101	89	113
ATP	85	109	19	105	105	90	46	112	94	95	5	111	77	110
Phosphonates
Aminoethyl phosphonic acid	0	1			1				0	3	0	0	8	13

p-NPP, para-nitrophenyl phosphate.