Figure 7.

Effect of docosahexaenoic acid-loaded liposomes (ω-liposomes) and control liposomes (C-liposomes) on lipopolysaccharide (LPS)-induced TLR4 activation.

Notes: Human HEK-Blue TLR4 cells were treated with the liposomes and stimulated with LPS for 18 hours. (A) TLR4 activation was monitored by measuring the secreted embryonic alkaline phosphatase (SEAP) in the medium calorimetrically. (B) Cell viability was assessed by MTS assay under the same experimental conditions. Data presented as mean ± standard error of mean from two independent experiments (each n$4). **P<0.01 compared to control nontreated (NT) LPS-stimulated cells (Student’s t-test). White bars indicate nontreated, nonstimulated control cells; black bars indicate stimulated control cells not treated with liposomal formulations.

Abbreviation: TL, total lipid.