Table 1.

Characteristics of liposomes

Liposomal formulation	Molar ratio (DHA:DPPC:Chol: DSPE-PEG2,000)	Mean diameter (nm)	Polydispersity index	ζ-Potential (mV)	Total lipid yield (%)	DHA-loading content (%)	DHA-entrapment efficiency (%)
ω-liposomes	1:0.85:1:0.15	99±16	0.06±0.01	−15.7±2.5	69±8	14.6±1.2	81.35±3.24
C-liposomes	0:1.85:1:0.15	100±17	0.08±0.03	−15.0±0.6	70±10	Not applicable	Not applicable

Notes: Data presented as ratio or mean ± SD (n=3).

$$\text{Total lipid yeild}(\%)=\frac{\text{Total lipid recovered}\left(\mu \text{mol}\right)}{\text{initial lipid used}\left(\mu \text{mol}\right)}\times 100\%,\text{Entrapment efficiency}\left(\%\right)=\frac{\text{Final drug/lipid ratio}}{\text{Initial drug/lipid ratio}}\times 100\%,\text{Loading content}\left(\%\right)=\frac{\text{Amount of DHA}\left(\text{mg}\right)}{\text{Amount of total lipid}\left(\text{mg}\right)}\times 100\%.$$

Abbreviations: DHA, docosahexaenoic acid; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; Chol, cholesterol; DSPE, distearoyl phosphatidylethanolamine; PEG, polyethylene glycol; ω-liposomes, docosahexaenoic acid-loaded liposomes. yield (%) Initial lipid used