Figure 1.

Proliferation of hiPSC-NS/PCs Treated with or without GSI

(A) Number of living cells counted in ten separate fields using a microscope and compared among each group (n = 5 independent experiments).

(B) Micrographs showing representative hiPSC-NS/PC aggregates for each group and cell line. Scale bars, 200 μm.

(C) Size of hiPSC-NS/PCs measured in ten separate fields using a microscope and compared among each group (n = 5 independent experiments).

(D) Cell-cycle analyses of hiPSC-NS/PCs performed using flow cytometry. Representative dot plots are shown for each group.

(E) Histograms show the relative distribution of hiPSC-NS/PCs across the different cell cycle phases under self-renewing conditions (n = 5 independent experiments).

(F) Analyses of hiPSC-NS/PC apoptosis performed using the annexin V/7-AAD apoptosis assay. The histograms show the relative distribution of hiPSC-NS/PCs across the different phases (n = 5 independent experiments).

^∗p < 0.05, ^∗∗p < 0.01, and not significant (N.S.) according to one-way ANOVA with the Tukey-Kramer test. Data are presented as means ± SEM.