Figure 3.

DNA Microarray and RT-PCR Analyses of GSI-Treated hiPSC-NS/PCs

(A) Venn diagrams showing the human genes with decreased expression in each group of GSI-treated 253G1 hiPSC-NS/PCs. Color key: red, 36 genes with decreased expression in the GSI-1d group; blue, 213 genes with decreased expression in the GSI-4d group; purple, 61 genes with decreased expression in both groups (n = 3 independent experiments).

(B) Venn diagrams showing the human genes with increased expression in each group of GSI-treated 253G1 hiPSC-NS/PCs. Color key: red, 5 genes with increased expression in the GSI-1d group; blue, 94 genes with increased expression in the GSI-4d group; purple, 53 genes with increased expression in both groups (n = 3 independent experiments).

(C) Venn diagrams showing the human genes with decreased expression in the GSI-1d and GSI-4d group of 836B3 hiPSC-NS/PCs. Color key: red, 23 genes with decreased expression in the GSI-1d group; blue, 37 genes with decreased expression in the GSI-4d group; purple, 26 genes with decreased expression in both groups (n = 3 independent experiments).

(D) Venn diagrams showing the human genes with increased expression in the GSI-1d and GSI-4d groups of 836B3 hiPSC-NS/PCs. Color key: red, 1 gene with increased expression in the GSI-1d group; blue, 70 genes with increased expression in the GSI-4d group; purple, 7 genes with increased expression in both groups (n = 3 independent experiments).

(E and F) (E) Lists showing the downregulated and (F) upregulated genes compared with control and GSI-1d and GSI-4d groups for each cell line using DNA microarrays (fold change >2.0).

(G) qRT-PCR analyses of known EMT-related genes in the GSI-1d and GSI-4d groups compared with the control group (equal to 1). The data were normalized to the reference GAPDH levels (n = 3 independent experiments). ^∗p < 0.05 and ^∗∗p < 0.01 according to one-way ANOVA with the Tukey-Kramer test. Data are presented as means ± SEM.

See also Figure S1.