Figure 7.

Long-Term Observations after Transplantation of GSI-Pretreated 201B7 hiPSC-NS/PCs

(A) Quantitative analyses of the photon counts derived from the transplanted cells until 89 days post transplantation (control group, n = 10 mice; GSI⁺ group, n = 10 mice).

(B) Representative images of mice at 0, 14, 28, 56, and 89 days after transplantation of 201B7 hiPSC-NS/PCs that were pretreated with or without GSI.

(C) Representative H&E-stained images of the spinal cords from each group at 89 days after transplantation. Scale bars, 1,000 μm.

(D) Representative immunohistological images of HNA⁺ cells, showing a graft from each group at 89 days after transplantation. Scale bars, 1,000 μm.

(E) Graft volumes of the hiPSC-NS/PCs were measured and estimated for each group of SCI model animals at 89 days after transplantation (control group, n = 10 mice; GSI⁺ group, n = 10 mice).

(F) Comparison of the BMS scores among the PBS, control, and GSI⁺ groups. Motor function in the hind limbs was assessed weekly for up to 89 days after transplantation using the BMS score (PBS group, n = 10; control group, n = 10; GSI⁺ group, n = 10 mice).

(G) Comparison of the rotarod test among the PBS, control, and GSI⁺ groups. The rotarod test was performed at 89 days after transplantation. Histograms show the total run time (PBS group, n = 10; control group, n = 10; GSI⁺ group, n = 10 mice).

(H) Comparison of the stride lengths among the PBS, control, and GSI⁺ groups. Treadmill gait analyses were performed at 89 days after transplantation using the DigiGait system. Histograms show the stride lengths (PBS group, n = 10; control group, n = 10; GSI⁺ group, n = 10 mice).

^∗p < 0.05 and not significant (N.S.) according to the Wilcoxon rank-sum test (A and E), two-way repeated-measures ANOVA with the Tukey-Kramer test (F), or one-way ANOVA with the Tukey-Kramer test (G and H). Data are presented as means ± SEM.