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. 2016 Sep 8;7(4):777–786. doi: 10.1016/j.stemcr.2016.08.003

Figure 1.

Figure 1

Cellular Characterization of DS-ESCs

Karyotype analysis was performed on all five DS cell lines.

(A) Metaphases of the five cell lines CSES13, 20, 21, 32, and 44 show a third copy of chromosome 21 in all lines (47,XX,+21 or 47,XY,+21). CSES32 and CSES44 cell lines were characterized for markers of pluripotent stem cells.

(B) CSES32 and CSES44 colonies stained positive for alkaline phosphatase.

(C) CSES32 and CSES44 colonies show positive staining for OCT4.

(D) All DS-ESC lines show expression of pluripotent genes such as NANOG, SOX2, and OCT4 similar to WT cells. The WT column represents the average of three different WT cell lines, and the DS column represents the average expression level of five different DS cell lines. Error bars represent SEM.

(E) CSES32 and CSES44 cell lines were differentiated in vivo by injecting them into immunodeficient mice to create teratomas. Teratoma sectioning and staining with H&E show differentiation into the three germ layers: EC marks ectoderm, ME marks mesoderm, and EN marks endoderm.