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. 2016 Oct 15;27(20):3005–3012. doi: 10.1091/mbc.E15-06-0423

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© 2016 Weerasinghe et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Resistance to hepatocyte apoptosis during culture is caspase dependent. (A) Hepatocytes were isolated from FVB/N and C3H/He mice and treated with FasL (0.5 μg/ml, 6 h) ± the caspase inhibitor Z-VAD-FMK (10 μM) after 3 h of cell attachment. Total cell lysates were analyzed by blotting for the indicated antigens. (B) Apoptotic cells were visualized using annexin-V staining (green), with counterstaining using DAPI (blue) to visualize nuclei. Scale bar, 400 μm. (C) Quantification of percentage of annexin-V⁺ cells in B.