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. 2016 Oct 15;27(20):3005–3012. doi: 10.1091/mbc.E15-06-0423

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© 2016 Weerasinghe et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — mRNA and lysosomal protein degradation contribute to the depletion of caspases 3 and 7 in short-term cultured hepatocytes. Hepatocytes were isolated from FVB/N mice, and cells were collected at the indicated times and then processed to obtain total mRNA and total cell lysates. (A) Relative expression of caspase 3 and 7 mRNA compared with the 0-h baseline levels. *p < 0.05. (B) Cell lysates were analyzed by blotting. (C, D) Hepatocytes were isolated from FVB/N mice (C) and C57BL/6 mice (D) and then cultured with or without the autophagy inhibitor 3MA (5 mM, C) and the lysosomal protease inhibitor leupeptin (Leu; 40 μM) or pepstatin-A (PA; 10 μM; C and D). Hepatocytes were collected after 9 h of treatment, and total cell lysates were analyzed by blotting. (E) Schematic summarizing the overall findings. Short-term culture of mouse hepatocyte results in marked down-regulation of caspases 3 and 7 but not caspase 8. This down-regulation is accompanied by resistance to Fas-medicated apoptosis in a manner dependent on mouse genetic background.