FIGURE 1:

TACC2 can act as a +TIP that localizes in front of EB1 in many embryonic cell types. (A–J) Representative images of mKate2-tubulin, GFP-TACC2, and merged in cultured embryonic mesenchymal cells derived from neural tube (A–C) and neuronal growth cone (F–H). See Supplemental Movies S1 and S2. (D, I) Magnified time-lapse montage of the boxed regions in C and H. The time interval between frames is ∼2–3 s. (E, J) Fluorescence intensity profile of GFP-TACC2. (K–T) Images of mKate2-EB1, GFP-TACC2, and merged in cultured embryonic mesenchymal cell (K–M) and neuronal growth cone (P–R). See Supplemental Movie S3. (N, S) Magnified time-lapse montage of the boxed region in M and R. For N, the green channel was translated left by ∼0.2 μm to account for the different acquisition times of the red and green channels (MT plus-end velocity ∼0.24 μm/s in this series, and the green channel was imaged 600 ms after the red channel). This allows for a more accurate visualization of +TIP colocalization. For S, the green channel was translated left by ∼0.1 μm (plus end was moving at 0.085 μm/s, and the green channel was imaged 600 ms after the red channel). For all fluorescence intensity profiles, ∼20 MTs for each condition were quantified by intensity line scans. The MT plus end is toward the right, and error bars represent SDs. Scale bars, 5 μm (montages), 10 μm (all others).