FIGURE 3:

TACC2 can regulate microtubule plus-end dynamics through its C-terminal domain in a cell type–specific manner. All raw data of automated tracking of mKate2-EB1 comets were normalized to the same-day control means, and all reported values are relative ones combined over at least three separate experiments. (A–C) Quantification of mean values of MT parameters (velocity, lifetime, length) upon TACC2 OE in embryonic mesenchymal cells. (D–F) MT parameters in growth cones (GC). (G–I) MT parameters in mesenchymal cells on overexpression of C- or N-terminal-truncated TACC2. (J–L) Quantification of MT parameters in mesenchymal cells when multiple combinations of TACC family members were expressed. Bars on dot plots show mean and SEM. To assess statistical significance, ANOVA tests were first performed (for multiple conditions), followed by an unpaired t test. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, n.s., not significant.