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. 2016 Oct 15;27(20):3143–3155. doi: 10.1091/mbc.E16-05-0272

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© 2016 Rodrigues et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Asap affects early-embryo Golgi organization. Side views and single cross-sections at or below the nuclei of control, Asap, Arf1, and AP-2α RNAi embryos expressing histone-mRFP for visualization of nuclei and stained for GM130 and LqfR for markers of the cis- and trans-Golgi, respectively (embryos at interphase). cis- and trans-Golgi compartments abnormally aggregate in Asap and Arf1 RNAi embryos (brackets in side views and inserts of single sections). AP-2α RNAi embryos display nuclear disorganization similar to that of Asap and Arf1 RNAi embryos but not the Golgi disorganization. Quantification of Golgi disruptions is presented below. N, embryo numbers (compiled from two or more experiments, each with similar results). Constructs expressed as in Figure 1.