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. 2016 Oct 15;27(20):3143–3155. doi: 10.1091/mbc.E16-05-0272

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© 2016 Rodrigues et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — An Asap-insensitive Arf1 construct is relatively ineffective for furrow ingression and Golgi organization. (A) 3D reconstructions of metaphase syncytial embryos expressing Arf1 RNAi with RNAi-resistant Arf1^[N52A] (left) or RNAi-resistant Arf1^[I46D] (right). Amph shows furrows, and pH3 shows cell cycle stage. Control Arf1^[N52A] considerably rescues Arf1 RNAi embryos, but Asap-insensitive Arf1^[I46D] does not. Below, xy-sections from the embryo surface to below the nuclei. Quantified to the right with Arf1 RNAi embryos coexpressing GFP as a control for the number of UAS constructs. N, number of metaphase embryos (compiled from two or more experiments, each with similar results). (B) Single cross-sections below the nuclei of arf1^shRNA2 Arf1^[N52A] (left) and arf1^shRNA2 Arf1^[I46D] (right) embryos stained for GM130 and LqfR (embryos at interphase). More abnormal Golgi aggregation was observed with the Asap-insensitive Arf1^[I46D] than the control Arf1^[N52A]. (A, B) Constructs expressed as in Figure 1.