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. 2016 Sep 15;7(4):619–634. doi: 10.1016/j.stemcr.2016.08.011

Figure 2.

Figure 2

Lymphocyte Reactions of Allogeneic iPSC-Derived RPE Cells: PBMC-RPE MLR Assay

(A) CFSE-labeled PBMCs with iPS-RPE cells were analyzed by flow cytometry. iPS-RPE cells (target cells, 454E2 from HLA homozygote donors) or B cells (a positive control) were co-cultured with PBMCs (effector cells) from a TLHD1 donor for 120 hr (effector/target ratio = 200:1, 100:1, or 50:1).

(B) RPE cells (454E2 iPS-RPE, TLHD1 iPS-RPE, and ARPE-19 cells) were co-cultured with PBMCs from a TLHD1 donor (left panels) or a TLHD21 donor (right panels). The TLHD21 donor was completely matched for HLA-A, -B, and -DRB1 in 454E2 iPS-RPE cells, and the TLHD1 donor was mismatched. Numbers in the histogram indicate CFSE-positive cells.