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Glutamine synthetase (GS) immunoreactivity density (arbitrary unit) in the liver among four age/diet groups of mice. In aged mice, the GS immunoreactivity density is higher with high-fat diet (HFD) than control diet (CD) feeding (**adjusted P = 0.004, Holm-Sidak's multiple comparison test following two-way analysis of variance). Data are expressed as mean and its 95% confidence interval.
