Table 1.
Primary antibodies used in immunohistochemistry (IHC) and western blot (WB).
| Antigen | Description of Immunogen | Source, Host Species, Cat. # Clone or Lot#, RRID |
Dilution |
|---|---|---|---|
| Zic2 | Amino acids 428–530 of human Zic2 (SSGYESSTPPGLVSPSAEPQSSSNLSPAA AAAAAAAAAAAAAVSAVHRGGGSGSG GAGGGSGGGSGSGGGGGGAGGGGGGS SGGGSGTAGGH SGLSSNFNEW) |
Brown et al. (2003), rabbit polyclonal, RRID: AB_2315623 |
1:10,000 (IHC) |
| Islet1/2 | C-terminus portion amino acids 178- 349 of rat Islet1 (Karlsson et al.,1990) (PEKTTRVRTVLNEKQLHTLRTCYAANPR PDALMKEQLVEMTGLSPRVIRVWFQNKR CKDKKRSIMMKQLQQQQPNDKTNIQGMT GTPMVAASPERHDGGLQANPVEVQSYQP PWKVLSDFALQSDIDQPAFQQLVNFSEGG PGSNSTGSEVASMSSQLPDTPNSMVASPIE A) |
Developmental Studies Hybridoma Bank, mouse monoclonal, contributed by T. Jessell, clone 39.4D5, RRID: AB_528173 |
1:100 (IHC) |
| Connexin 43 | C-terminus portion amino acids 332- 382 of human Connexin 43, Accession# P17302 (AQPFDFPDDNQNSKKLAAGHEL QPLAIVDQRPSSRASSRASSRPRP DDLEI) |
Santa Cruz Biotechnology, goat polyclonal, Cat# sc-6560, clone C-20, RRID: AB_638639 |
1:200 (IHC) |
| Na+K+- ATPase |
Recombinant protein corresponding to amino acids 551–850 of human Na+/K+-ATPase a1 |
Santa Cruz Biotechnology, rabbit polyclonal, Cat# sc- 28800, RRID: AB_2290063 |
1:50 (IHC) |
| ZO-1 | Synthetic peptide in the middle region (amino acids 1000–1150) of the ZO-1 protein |
Thermo Fisher Scientific, rabbit polyclonal, Cat# 40–2200, RRID: AB_10104693 |
1:200 (IHC) |
| Otx2 | Recombinant protein corresponding to Met1-Leu289 of human Otx2 Accession # P32243 |
R and D Systems, goat polyclonal, Cat# AF1979, RRID: AB_2157172 |
1:200 (IHC) |
| P-cadherin | Mouse endoderm cell line PSA5-E. Recognizes the 118 kDa P-cadherin by immunoblot (Nose and Takeichi, 1986) |
Invitrogen, rat monoclonal, Cat# 132000Z, clone PCD-1, RRID: AB_86574 |
1:500 (IHC) |
| Mitf | N-terminal Taq-Sac fragment of human Mi protein (corresponding to amino acids 15–290) |
Thermo Scientific, mouse monoclonal Cat# MS-771-P0, clone C5, RRID: AB_141540 |
1:500 (IHC) |
| Vinculin | Full length human vinculin from human uterus. Accession #P18206 : recognized the 116 kDa vinculin by immunoblot |
Sigma-Aldrich, mouse, monoclonal, Cat# V9131, clone hVIN-1, RRID: AB_477629 |
1:1000 (WB) |
| Connexin 43 | Synthetic peptide corresponding to the C-terminal segment of the cytoplasmic domain (amino acids 363–382 with N-terminally added lysine) of human/rat connexin43 (PSSRASSRASSRPRPDDLEI) |
Sigma-Aldrich, rabbit polyclonal, Cat# C6219, RRID: AB_476857 |
1:6000 (WB) |
| Phospho- Connexin 43 (Ser368) |
Synthetic phosphopeptide corresponding to residues surrounding Ser368 of human connexin 43 (RPSSRAsSRASS) |
Cell Signaling Technology, rabbit polyclonal, Cat# 3511S, RRID: AB_2110169 |
1:100 (WB) |