Figure 3.

Graphical comparison of ligand-associated spectral shifts of acrylodan with ligand-associated variability of distances (Δ distance) to the active serine observed from X-ray structures (cf. Fig. 2; Table 1). Absolute values of magnitude changes in both spectral shifts and Δ distance are represented as the percentage of total change observed at each of the acrylodan-labeling sites to facilitate their comparison. Adapted from data published in Refs. 12 and 13.