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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Anal Bioanal Chem. 2016 Aug 22;408(27):7745–7751. doi: 10.1007/s00216-016-9871-7

Figure 1.

Figure 1

A. EtBr accumulation assay of strains DL41 (black open circles), DL41ΔacrB-pQE70 (black filled circles), and DL41ΔacrB -pQE70-AcrB (red circles). The plasmid-encoded AcrB is functional in the knockout strain and effectively eliminates the accumulation of EtBr into the cytosol. B. EtBr accumulation assay of strains DL41ΔacrB-pQE70 (top two traces) and DL41ΔacrB -pQE70-AcrB (bottom two traces) cultured in the presence of Met (red) or AHA (black). The replacement of Met by AHA does not have a dramatic effect on the efflux of EtBr by AcrB. C. Growth of DL41 in the M9 media containing 19 essential amino acid without Met (squares), supplemented with Met (diamond), or supplemented with AHA (triangle).