Figure 7.

Hepatic expression of GH. GH mRNA from the pituitary and liver (partially hepatectomized rats) was retrotranscripted with specific primers and the resultant cDNAs were resolved in 2% agarose and stained with ethidium bromide, before and after using the enzyme of restriction RsaI. As the figure shows pituitary (pit) and liver (liv), GH was detected with the expected molecular weight: 328 bp, because the primers used flanked a region situated between exons 4 and 5 of rat GH gene. The bands obtained after cutting the main GH amplified with RsaI led to the appearance of two bands in the molecular weight expected (238 and 90 bp), both in pituitary and liver GH. Black arrows indicate the main GH product. Blue arrows indicate the products obtained after cutting with RsaI. Reprinted with permission of the publisher. Source: J Devesa, Devesa P, Reimunde P. Growth hormone: actions and preventive and therapeutic applications. Med Clin (Barc) 2010; 135 (14): 665-670. Copyright © 2009 Elsevier Spain, S.L. All rights reserved.

Abbreviations: MWM, molecular weight markers; bp, basepairs.