Figure 1.
Protective effect of yeasts Debaryomyces hansenii (Dh97) and Yarrowia lipolytica (Yl242) against a V. anguillarum (Va) challenge in zebrafish larvae. Larvae were inoculated with different concentration (104–107 CFU/mL) of Dh97 (A,B) and Yl242 (C,D) on day 4 dpf, and challenged with V. anguillarum on day 5 dpf. Survival rate (%) of conventionally raised larvae (CONV-R) (A,C), and germ-free larvae (GF) (B,D) at 4 days post- V. anguillarum inoculation. The results show the mean ± SD of 3 independent experiments with three replicates each. Different letters indicate statistically significant differences among groups (Kaplan Meier, Wilcoxon P < 0.003).