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. 2016 Oct 14;6:127. doi: 10.3389/fcimb.2016.00127

Figure 2.

Figure 2

Expression of innate immune genes analyzed by qPCR in conventionally raised (CONV-R) larvae challenged with V. anguillarum at 5 dpf, pre-treated at 4 dpf with different concentrations of each yeast, relative to non-challenged CONV-R larvae. Dh97, Debaryomyces hansenii 97; Yl242, Yarrowia lipolytica 242; il1b, interleukin 1 beta; tnfa, tumor necrosis factor a; c3, complement component 3; mpx, myeloid-specific peroxidase; il10, interleukin 10; hpc, hours post V. anguillarum-challenge. Data were normalized to beta actin 1. The results show the mean ± SD of 3 independent experiments with three replicates each. *Indicates statistically significant differences of the experimental groups with non-challenged CONV-R larvae, †Indicates statistically significant differences of the V. anguillarum-challenged larvae treated with yeasts (CONV-R + yeast + Va) with V. anguillarum-challenged CONV-R larvae (CONV-R + Va).