Table 1.
Primer sets used in qPCR
| Gene name | Forward primer (5′‐ 3′) | Reverse primer (5′‐ 3′) | Product size (bp) | Accession number |
|---|---|---|---|---|
| Kcnma1 | CAGTTTGACCACAACGCTGG | TGTGGGTACTCATGGGCTTG | 233 | NM001253378 |
| Kcnmb1 | CTCAACAGGTCCCTATCCATCT | CCAAAGTCACTGGGAATCGGA | 200 | NM031169 |
| Cacna1c | CGTTCTCATCCTGCTCAACA | GGTGTACCTCGGTGATTGCT | 236 | BC138031 |
| Cacna1g | TCATAGCCGTGCTGATGAAG | AAGGGAGAAGCCTGAAGAGG | 236 | NM009783.3 |
| Cacna1h | CCTTTCTCAGCGTCTCCAAC | GCCACAATGATGTCAACCAG | 169 | BC138026 |
| Cacna1a | TGCTGTGCTCACTGTTTTCC | TTCTCCACACGTTCCCTTTC | 198 | NM007578 |
| Trpc1 | GACATTCCAGGTTTCGTCTTG | GTCAGCACTAAGTTCAAACGC | 107 | NM011643 |
| Trpc3 | GATCGAGGATGACAGTGATGTAG | TCCATCATCGAAGTAGGAGAGC | 73 | NM019510 |
| Trpc6 | TACAATCTGGCCAGGATAAAGTG | CACAGCGATTGCATAAAGACC | 75 | NM013838 |
| Actb | AGCCATGTACGTAGCCATCC | CTCTCAGCTGTGGTGGTGAA | 228 | NM007393 |